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Complete genome of a dengue virus serotype 4 strain from Amazonas, Brazil.


ABSTRACT: Dengue virus (DENV) infections represent a significant concern for public health worldwide, being considered as the most prevalent arthropod-borne virus regarding the number of reported cases. In this study, we report the complete genome sequencing of a DENV serotype 4 isolate, genotype II, obtained in the city of Manaus, directly from the serum sample, applying Ion Torrent sequencing technology. The use of a massive sequencing technology allowed the detection of two variable sites, one in the coding region for the viral envelope protein and the other in the nonstructural 1 coding region within viral populations.

SUBMITTER: Nascimento VA 

PROVIDER: S-EPMC4750455 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Complete genome of a dengue virus serotype 4 strain from Amazonas, Brazil.

Nascimento Valdinete Alves do VA   Souza Victor Costa de VC   Naveca Felipe Gomes FG  

Memorias do Instituto Oswaldo Cruz 20160202 2


Dengue virus (DENV) infections represent a significant concern for public health worldwide, being considered as the most prevalent arthropod-borne virus regarding the number of reported cases. In this study, we report the complete genome sequencing of a DENV serotype 4 isolate, genotype II, obtained in the city of Manaus, directly from the serum sample, applying Ion Torrent sequencing technology. The use of a massive sequencing technology allowed the detection of two variable sites, one in the c  ...[more]

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