Red Sea Atlantis II brine pool nitrilase with unique thermostability profile and heavy metal tolerance.

ABSTRACT:

Background

Nitrilases, which hydrolyze nitriles in a one-step reaction into carboxylic acids and ammonia, gained increasing attention because of the abundance of nitrile compounds in nature and their use in fine chemicals and pharmaceutics. Extreme environments are potential habitats for the isolation and characterization of extremozymes including nitrilases with unique resistant properties. The Red Sea brine pools are characterized by multitude of extreme conditions. The Lower Convective Layer (LCL) of the Atlantis II Deep Brine Pool in the Red Sea is characterized by elevated temperature (68 °C), high salt concentrations (250 ‰), anoxic conditions and high heavy metal concentrations.

Results

We identified and isolated a nitrilase from the Atlantis II Deep Brine Pool in the

SUBMITTER: Sonbol SA 

PROVIDER: S-EPMC4751646 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature