Oxidative changes and signalling pathways are pivotal in initiating age-related changes in articular cartilage.

ABSTRACT:

Objective

To use a computational approach to investigate the cellular and extracellular matrix changes that occur with age in the knee joints of mice.

Methods

Knee joints from an inbred C57/BL1/6 (ICRFa) mouse colony were harvested at 3-30 months of age. Sections were stained with H&E, Safranin-O, Picro-sirius red and antibodies to matrix metalloproteinase-13 (MMP-13), nitrotyrosine, LC-3B, Bcl-2, and cleaved type II collagen used for immunohistochemistry. Based on this and other data from the literature, a computer simulation model was built using the Systems Biology Markup Language using an iterative approach of data analysis and modelling. Individual parameters were subsequently altered to assess their effect on the model.

Results

A progressive loss of cartilage ma

SUBMITTER: Hui W 

PROVIDER: S-EPMC4752670 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature