Actin Dynamics Regulates Dendritic Cell-Mediated Transfer of HIV-1 to T Cells.

ABSTRACT: Whereas human dendritic cells (DCs) are largely resistant to productive infection with HIV-1, they have a unique ability to take up the virus and transmit it efficiently to T lymphocytes through a process of trans-infection or trans-enhancement. To elucidate the molecular and cell biological mechanism for trans-enhancement, we performed an shRNA screen of several hundred genes involved in organelle and membrane trafficking in immature human monocyte-derived dendritic cells (MDDCs). We identified TSPAN7 and DNM2, which control actin nucleation and stabilization, as having important and distinct roles in limiting HIV-1 endocytosis and in maintaining virus particles on dendrites, which is required for efficient transfer to T lymphocytes. Further characterization of this process may provide in

SUBMITTER: Menager MM 

PROVIDER: S-EPMC4752894 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature