Unknown

Dataset Information

0

Uncoupling complement C1s activation from C1q binding in apoptotic cell phagocytosis and immunosuppressive capacity.


ABSTRACT: Complement activation contributes to inflammation in many diseases, yet it also supports physiologic apoptotic cells (AC) clearance and its downstream immunosuppressive effects. The roles of individual complement components in AC phagocytosis have been difficult to dissect with artificially depleted sera. Using human in vitro systems and the novel antibody complement C1s inhibitor TNT003, we uncoupled the role of the enzymatic activation of the classical pathway from the opsonizing role of C1q in mediating a) the phagocytosis of early and late AC, and b) the immunosuppressive capacity of early AC. We found that C1s inhibition had a small impact on the physiologic clearance of early AC, leaving their immunosuppressive properties entirely unaffected, while mainly inhibiting the phagocytosis of late apoptotic/secondary necrotic cells. Our data suggest that C1s inhibition may represent a valuable therapeutic strategy to control classical pathway activation without causing significant AC accumulation in diseases without defects in AC phagocytosis.

SUBMITTER: Colonna L 

PROVIDER: S-EPMC4753131 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Uncoupling complement C1s activation from C1q binding in apoptotic cell phagocytosis and immunosuppressive capacity.

Colonna Lucrezia L   Parry Graham C GC   Panicker Sandip S   Elkon Keith B KB  

Clinical immunology (Orlando, Fla.) 20160106


Complement activation contributes to inflammation in many diseases, yet it also supports physiologic apoptotic cells (AC) clearance and its downstream immunosuppressive effects. The roles of individual complement components in AC phagocytosis have been difficult to dissect with artificially depleted sera. Using human in vitro systems and the novel antibody complement C1s inhibitor TNT003, we uncoupled the role of the enzymatic activation of the classical pathway from the opsonizing role of C1q i  ...[more]

Similar Datasets

| S-EPMC3666734 | biostudies-literature
| S-EPMC10723154 | biostudies-literature
| S-EPMC2449358 | biostudies-literature
| S-EPMC3752233 | biostudies-literature
2012-05-02 | GSE30177 | GEO
| S-EPMC5067173 | biostudies-literature
| S-EPMC7609443 | biostudies-literature
| S-EPMC7313146 | biostudies-literature
| S-EPMC5789954 | biostudies-literature
| S-EPMC4654252 | biostudies-literature