Water-deficiency conditions differently modulate the methylome of roots and leaves in barley (Hordeum vulgare L.).

ABSTRACT: One of the strategies of plant adaptation to stress is the modulation of gene expression, which may result from the regulation of DNA methylation. This study attempted to characterize and compare the barley methylome of leaves and roots under water-deficiency treatment and in the subsequent rewatering phase. Our results, obtained using methylation-sensitive amplification polymorphism sequencing analysis, indicated that the overall DNA methylation level in the barley genome was high and in general stable under water-deficiency conditions. Nevertheless, numerous differentially methylated sites (DMSs) were induced by stress in the leaves and roots. Equal proportions of novel stress-induced methylation and demethylation events were observed within the genes in the leaves, but new methylations

SUBMITTER: Chwialkowska K 

PROVIDER: S-EPMC4753852 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature