Enhanced production of thermostable amidase from Geobacillus subterraneus RL-2a MTCC 11502 via optimization of physicochemical parameters using Taguchi DOE methodology.

ABSTRACT: The specific effect of chemical and physical factors on amidase production from Geobacillus subterraneus RL-2a was investigated using design of experiments (DOE) methodology. The one-factor-at-a-time (OFAT) method was used to study the effects of carbon and nitrogen sources on amidase production. Subsequently, optimal levels of physical parameters and key media components, namely temperature, pH, sucrose, K₂HPO₄, NaCl, yeast, CaCl₂·2H₂O and MgSO₄·7H₂O, were determined using the Taguchi orthogonal array (OA) experimental design (DOE) methodology. Taguchi method based on three levels with a OA layout of L18 (2¹ × 3⁷) with eight most influential factors on amidase synthesis for the proposed experimental de

SUBMITTER: Mehta PK 

PROVIDER: S-EPMC4754296 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature