Unknown

Dataset Information

0

SIRT7-dependent deacetylation of the U3-55k protein controls pre-rRNA processing.


ABSTRACT: SIRT7 is an NAD(+)-dependent protein deacetylase with important roles in ribosome biogenesis and cell proliferation. Previous studies have established that SIRT7 is associated with RNA polymerase I, interacts with pre-ribosomal RNA (rRNA) and promotes rRNA synthesis. Here we show that SIRT7 is also associated with small nucleolar RNP (snoRNPs) that are involved in pre-rRNA processing and rRNA maturation. Knockdown of SIRT7 impairs U3 snoRNA dependent early cleavage steps that are necessary for generation of 18S rRNA. Mechanistically, SIRT7 deacetylates U3-55k, a core component of the U3 snoRNP complex, and reversible acetylation of U3-55k modulates the association of U3-55k with U3 snoRNA. Deacetylation by SIRT7 enhances U3-55k binding to U3 snoRNA, which is a prerequisite for pre-rRNA processing. Under stress conditions, SIRT7 is released from nucleoli, leading to hyperacetylation of U3-55k and attenuation of pre-rRNA processing. The results reveal a multifaceted role of SIRT7 in ribosome biogenesis, regulating both transcription and processing of rRNA.

SUBMITTER: Chen S 

PROVIDER: S-EPMC4754350 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

altmetric image

Publications

SIRT7-dependent deacetylation of the U3-55k protein controls pre-rRNA processing.

Chen Sifan S   Blank Maximilian Felix MF   Iyer Aishwarya A   Huang Bingding B   Wang Lin L   Grummt Ingrid I   Voit Renate R  

Nature communications 20160212


SIRT7 is an NAD(+)-dependent protein deacetylase with important roles in ribosome biogenesis and cell proliferation. Previous studies have established that SIRT7 is associated with RNA polymerase I, interacts with pre-ribosomal RNA (rRNA) and promotes rRNA synthesis. Here we show that SIRT7 is also associated with small nucleolar RNP (snoRNPs) that are involved in pre-rRNA processing and rRNA maturation. Knockdown of SIRT7 impairs U3 snoRNA dependent early cleavage steps that are necessary for g  ...[more]

Similar Datasets

2016-01-07 | E-GEOD-76578 | biostudies-arrayexpress
2016-01-07 | GSE76578 | GEO
| S-EPMC7144924 | biostudies-literature
| S-EPMC110750 | biostudies-literature
2018-12-12 | GSE115360 | GEO
| S-EPMC140510 | biostudies-literature
| S-EPMC5909432 | biostudies-literature
| S-EPMC5312328 | biostudies-literature
| S-EPMC5389538 | biostudies-literature
| S-EPMC84386 | biostudies-literature