Unknown

Dataset Information

0

The S-layer Protein DR_2577 Binds Deinoxanthin and under Desiccation Conditions Protects against UV-Radiation in Deinococcus radiodurans.


ABSTRACT: Deinococcus radiodurans has the puzzling ability to withstand over a broad range of extreme conditions including high doses of ultraviolet radiation and deep desiccation. This bacterium is surrounded by a surface layer (S-layer) built of a regular repetition of several proteins, assembled to form a paracrystalline structure. Here we report that the deletion of a main constituent of this S-layer, the gene DR_2577, causes a decrease in the UVC resistance, especially in desiccated cells. Moreover, we show that the DR_2577 protein binds the carotenoid deinoxanthin, a strong protective antioxidant specific of this bacterium. A further spectroscopical characterization of the deinoxanthin-DR_2577 complex revealed features which could suggest a protective role of DR_2577. We propose that, especially under desiccation, the S-layer shields the bacterium from incident ultraviolet light and could behave as a first lane of defense against UV radiation.

SUBMITTER: Farci D 

PROVIDER: S-EPMC4754619 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

altmetric image

Publications

The S-layer Protein DR_2577 Binds Deinoxanthin and under Desiccation Conditions Protects against UV-Radiation in Deinococcus radiodurans.

Farci Domenica D   Slavov Chavdar C   Tramontano Enzo E   Piano Dario D  

Frontiers in microbiology 20160216


Deinococcus radiodurans has the puzzling ability to withstand over a broad range of extreme conditions including high doses of ultraviolet radiation and deep desiccation. This bacterium is surrounded by a surface layer (S-layer) built of a regular repetition of several proteins, assembled to form a paracrystalline structure. Here we report that the deletion of a main constituent of this S-layer, the gene DR_2577, causes a decrease in the UVC resistance, especially in desiccated cells. Moreover,  ...[more]

Similar Datasets

| S-EPMC8533742 | biostudies-literature
2021-10-27 | GSE175875 | GEO
| S-EPMC2775278 | biostudies-literature
| S-EPMC153069 | biostudies-literature
| S-EPMC4325154 | biostudies-literature
| S-EPMC5009958 | biostudies-literature
| S-EPMC5570939 | biostudies-literature
| S-EPMC2755865 | biostudies-literature
| S-EPMC5574771 | biostudies-literature
| S-EPMC8213827 | biostudies-literature