Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning.
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ABSTRACT:
SUBMITTER: Ozkumur AY
PROVIDER: S-EPMC4754792 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Publications
Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning.
Ozkumur Ayca Yalcin AY Goods Brittany A BA Love J Christopher JC
Small (Weinheim an der Bergstrasse, Germany) 20150629 36
Living-cell-based screens can facilitate lead discovery of functional therapeutics of interest. A versatile and scalable method is reported that uses dense arrays of nanowells for imparting defined patterns on monolayers of cells. It is shown that this approach can coordinate a multi-component biological assay by designing and implementing a high-throughput, functional nanoliter-scale neutralization assay to identify neutralizing antibodies against HIV. ...[more]
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