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Expanding the Landscape of Diterpene Structural Diversity through Stereochemically Controlled Combinatorial Biosynthesis.

ABSTRACT: Plant-derived diterpenoids serve as important pharmaceuticals, food additives, and fragrances, yet their low natural abundance and high structural complexity limits their broader industrial utilization. By mimicking the modularity of diterpene biosynthesis in plants, we constructed 51 functional combinations of class?I and II diterpene synthases, 41 of which are "new-to-nature". Stereoselective biosynthesis of over 50 diterpene skeletons was demonstrated, including natural variants and novel enantiomeric or diastereomeric counterparts. Scalable biotechnological production for four industrially relevant targets was accomplished in engineered strains of Saccharomyces cerevisiae.

SUBMITTER: Andersen-Ranberg J

PROVIDER: S-EPMC4755150 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Expanding the Landscape of Diterpene Structural Diversity through Stereochemically Controlled Combinatorial Biosynthesis.

Andersen-Ranberg Johan J Kongstad Kenneth Thermann KT Nielsen Morten Thrane MT Jensen Niels Bjerg NB Pateraki Irini I Bach Søren Spanner SS Hamberger Britta B Zerbe Philipp P Staerk Dan D Bohlmann Jörg J Møller Birger Lindberg BL Hamberger Björn B

Angewandte Chemie (International ed. in English) 20160108 6

Plant-derived diterpenoids serve as important pharmaceuticals, food additives, and fragrances, yet their low natural abundance and high structural complexity limits their broader industrial utilization. By mimicking the modularity of diterpene biosynthesis in plants, we constructed 51 functional combinations of class I and II diterpene synthases, 41 of which are "new-to-nature". Stereoselective biosynthesis of over 50 diterpene skeletons was demonstrated, including natural variants and novel ena ...[more]

PMID: 26749264

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