Widespread RNA binding by chromatin-associated proteins.

ABSTRACT:

Background

Recent evidence suggests that RNA interaction can regulate the activity and localization of chromatin-associated proteins. However, it is unknown if these observations are specialized instances for a few key RNAs and chromatin factors in specific contexts, or a general mechanism underlying the establishment of chromatin state and regulation of gene expression.

Results

Here, we perform formaldehyde RNA immunoprecipitation (fRIP-Seq) to survey the RNA associated with a panel of 24 chromatin regulators and traditional RNA binding proteins. For each protein that reproducibly bound measurable quantities of bulk RNA (90% of the panel), we detect enrichment for hundreds to thousands of both noncoding and mRNA transcripts.

Conclusion

For each protein, we find that

SUBMITTER: G Hendrickson D 

PROVIDER: S-EPMC4756407 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature