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FACS-style detection for real-time cell viscoelastic cytometry.

ABSTRACT: Cell mechanical properties have been established as a label-free biophysical marker of cell viability and health; however, real-time methods with significant throughput for accurately and non-destructively measuring these properties remain widely unavailable. Without appropriate labels for use with fluorescence activated cell sorters (FACS), easily implemented real-time technology for tracking cell-level mechanical properties remains a current need. Employing modulated optical forces and enabled by a low-dimensional FACS-style detection method introduced here, we present a viscoelasticity cytometer (VC) capable of real-time and continuous measurements. We demonstrate the utility of this approach by tracking the high-frequency cell physical properties of populations of chemically-modified cells at rates of ~ 1 s-1 and explain observations within the context of a simple theoretical model.

SUBMITTER: Kasukurti A

PROVIDER: S-EPMC4756765 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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FACS-style detection for real-time cell viscoelastic cytometry.

Kasukurti A A Eggleton C D CD Desai S A SA Marr D W M DW

RSC advances 20151202 128

Cell mechanical properties have been established as a label-free biophysical marker of cell viability and health; however, real-time methods with significant throughput for accurately and non-destructively measuring these properties remain widely unavailable. Without appropriate labels for use with fluorescence activated cell sorters (FACS), easily implemented real-time technology for tracking cell-level mechanical properties remains a current need. Employing modulated optical forces and enabled ...[more]

PMID: 26900453

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Dataset Information

FACS-style detection for real-time cell viscoelastic cytometry.

Publications

FACS-style detection for real-time cell viscoelastic cytometry.

Similar Datasets

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