Locked and proteolysis-based transcription activator-like effector (TALE) regulation.

ABSTRACT: Development of orthogonal, designable and adjustable transcriptional regulators is an important goal of synthetic biology. Their activity has been typically modulated through stimulus-induced oligomerization or interaction between the DNA-binding and activation/repression domain. We exploited a feature of the designable Transcription activator-like effector (TALE) DNA-binding domain that it winds around the DNA which allows to topologically prevent it from binding by intramolecular cyclization. This new approach was investigated through noncovalent ligand-induced cyclization or through a covalent split intein cyclization strategy, where the topological inhibition of DNA binding by cyclization and its restoration by a proteolytic release of the topologic constraint was expected. We show tha

SUBMITTER: Lonzaric J 

PROVIDER: S-EPMC4756844 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature