Modular Synthetic Inverters from Zinc Finger Proteins and Small RNAs.
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ABSTRACT:
SUBMITTER: Hsia J
PROVIDER: S-EPMC4757538 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Publications
Modular Synthetic Inverters from Zinc Finger Proteins and Small RNAs.
Hsia Justin J Holtz William J WJ Maharbiz Michel M MM Arcak Murat M Keasling Jay D JD
PloS one 20160217 2
Synthetic zinc finger proteins (ZFPs) can be created to target promoter DNA sequences, repressing transcription. The binding of small RNA (sRNA) to ZFP mRNA creates an ultrasensitive response to generate higher effective Hill coefficients. Here we combined three "off the shelf" ZFPs and three sRNAs to create new modular inverters in E. coli and quantify their behavior using induction fold. We found a general ordering of the effects of the ZFPs and sRNAs on induction fold that mostly held true wh ...[more]
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