Ontology highlight
ABSTRACT:
SUBMITTER: ElSohly AM
PROVIDER: S-EPMC4758138 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
ElSohly Adel M AM Netirojjanakul Chawita C Aanei Ioana L IL Jager Astraea A Bendall Sean C SC Farkas Michelle E ME Nolan Garry P GP Francis Matthew B MB
Bioconjugate chemistry 20150706 8
The present study describes an efficient and reliable method for the preparation of MS2 viral capsids that are synthetically modified with antibodies using a rapid oxidative coupling strategy. The overall protocol delivers conjugates in high yields and recoveries, requires a minimal excess of antibody to achieve modification of more than 95% of capsids, and can be completed in a short period of time. Antibody-capsid conjugates targeting extracellular receptors on human breast cancer cell lines w ...[more]