Ontology highlight
ABSTRACT:
SUBMITTER: Hoopes JI
PROVIDER: S-EPMC4758883 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Hoopes James I JI Cortez Luis M LM Mertz Tony M TM Malc Ewa P EP Mieczkowski Piotr A PA Roberts Steven A SA
Cell reports 20160128 6
APOBEC family cytidine deaminases have recently been implicated as powerful mutators of cancer genomes. How APOBECs, which are ssDNA-specific enzymes, gain access to chromosomal DNA is unclear. To ascertain the chromosomal ssDNA substrates of the APOBECs, we expressed APOBEC3A and APOBEC3B, the two most probable APOBECs mediating cancer mutagenesis, in a yeast model system. We demonstrate, using mutation reporters and whole genome sequencing, that APOBEC3A- and APOBEC3B-induced mutagenesis prima ...[more]