Unknown

Dataset Information

0

Regulation of Transcription Factor Yin Yang 1 by SET7/9-mediated Lysine Methylation.


ABSTRACT: Yin Yang 1 (YY1) is a multifunctional transcription factor shown to be critical in a variety of biological processes. Although it is regulated by multiple types of post-translational modifications (PTMs), whether YY1 is methylated, which enzyme methylates YY1, and hence the functional significance of YY1 methylation remains completely unknown. Here we reported the first methyltransferase, SET7/9 (KMT7), capable of methylating YY1 at two highly conserved lysine (K) residues, K173 and K411, located in two distinct domains, one in the central glycine-rich region and the other in the very carboxyl-terminus. Functional studies revealed that SET7/9-mediated YY1 methylation regulated YY1 DNA-binding activity both in vitro and at specific genomic loci in cultured cells. Consistently, SET7/9-mediated YY1 methylation was shown to involve in YY1-regulated gene transcription and cell proliferation. Our findings revealed a novel regulatory strategy, methylation by lysine methyltransferase, imposed on YY1 protein, and linked YY1 methylation with its biological functions.

SUBMITTER: Zhang WJ 

PROVIDER: S-EPMC4763200 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications


Yin Yang 1 (YY1) is a multifunctional transcription factor shown to be critical in a variety of biological processes. Although it is regulated by multiple types of post-translational modifications (PTMs), whether YY1 is methylated, which enzyme methylates YY1, and hence the functional significance of YY1 methylation remains completely unknown. Here we reported the first methyltransferase, SET7/9 (KMT7), capable of methylating YY1 at two highly conserved lysine (K) residues, K173 and K411, locate  ...[more]

Similar Datasets

2017-02-13 | GSE69759 | GEO
2017-02-13 | GSE69757 | GEO
2017-02-13 | GSE69758 | GEO
| PRJNA286699 | ENA
| S-EPMC2654809 | biostudies-literature
| S-EPMC4657790 | biostudies-literature
| S-EPMC10242864 | biostudies-literature
| S-EPMC9123262 | biostudies-literature
| S-EPMC2034312 | biostudies-literature
| S-EPMC6481341 | biostudies-literature