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T cell-specific inactivation of mouse CD2 by CRISPR/Cas9.

ABSTRACT: The CRISPR/Cas9 system can be used to mutate target sequences by introduction of double-strand breaks followed by imprecise repair. To test its use for conditional gene editing we generated mice transgenic for CD4 promoter-driven Cas9 combined with guide RNA targeting CD2. We found that within CD4(+) and CD8(+) lymphocytes from lymph nodes and spleen 1% and 0.6% were not expressing CD2, respectively. T cells lacking CD2 carryied mutations, which confirmed that Cas9 driven by cell-type specific promoters can edit genes in the mouse and may thus allow targeted studies of gene function in vivo.

SUBMITTER: Beil-Wagner J

PROVIDER: S-EPMC4763270 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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T cell-specific inactivation of mouse CD2 by CRISPR/Cas9.

Beil-Wagner Jane J Dössinger Georg G Schober Kilian K vom Berg Johannes J Tresch Achim A Grandl Martina M Palle Pushpalatha P Mair Florian F Gerhard Markus M Becher Burkhard B Busch Dirk H DH Buch Thorsten T

Scientific reports 20160223

The CRISPR/Cas9 system can be used to mutate target sequences by introduction of double-strand breaks followed by imprecise repair. To test its use for conditional gene editing we generated mice transgenic for CD4 promoter-driven Cas9 combined with guide RNA targeting CD2. We found that within CD4(+) and CD8(+) lymphocytes from lymph nodes and spleen 1% and 0.6% were not expressing CD2, respectively. T cells lacking CD2 carryied mutations, which confirmed that Cas9 driven by cell-type specific p ...[more]

PMID: 26903281

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