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A Proteomic Approach Identifies Candidate Early Biomarkers to Predict Severe Dengue in Children.

ABSTRACT: BACKGROUND:Severe dengue with severe plasma leakage (SD-SPL) is the most frequent of dengue severe form. Plasma biomarkers for early predictive diagnosis of SD-SPL are required in the primary clinics for the prevention of dengue death. METHODOLOGY:Among 63 confirmed dengue pediatric patients recruited, hospital based longitudinal study detected six SD-SPL and ten dengue with warning sign (DWS). To identify the specific proteins increased or decreased in the SD-SPL plasma obtained 6-48 hours before the shock compared with the DWS, the isobaric tags for relative and absolute quantification (iTRAQ) technology was performed using four patients each group. Validation was undertaken in 6 SD-SPL and 10 DWS patients. PRINCIPAL FINDINGS:Nineteen plasma proteins exhibited significantly different relative concentrations (p<0.05), with five over-expressed and fourteen under-expressed in SD-SPL compared with DWS. The individual protein was classified to either blood coagulation, vascular regulation, cellular transport-related processes or immune response. The immunoblot quantification showed angiotensinogen and antithrombin III significantly increased in SD-SPL whole plasma of early stage compared with DWS subjects. Even using this small number of samples, antithrombin III predicted SD-SPL before shock occurrence with accuracy. CONCLUSION:Proteins identified here may serve as candidate predictive markers to diagnose SD-SPL for timely clinical management. Since the number of subjects are small, so further studies are needed to confirm all these biomarkers.

SUBMITTER: Nhi DM

PROVIDER: S-EPMC4764501 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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A Proteomic Approach Identifies Candidate Early Biomarkers to Predict Severe Dengue in Children.

Nhi Dang My DM Huy Nguyen Tien NT Ohyama Kaname K Kimura Daisuke D Lan Nguyen Thi Phuong NT Uchida Leo L Thuong Nguyen Van NV Nhon Cao Thi My CT Phuc Le Hong le H Mai Nguyen Thi NT Mizukami Shusaku S Bao Lam Quoc LQ Doan Nguyen Ngoc NN Binh Nguyen Van Thanh NV Quang Luong Chan LC Karbwang Juntra J Yui Katsuyuki K Morita Kouichi K Huong Vu Thi Que VT Hirayama Kenji K

PLoS neglected tropical diseases 20160219 2

<h4>Background</h4>Severe dengue with severe plasma leakage (SD-SPL) is the most frequent of dengue severe form. Plasma biomarkers for early predictive diagnosis of SD-SPL are required in the primary clinics for the prevention of dengue death.<h4>Methodology</h4>Among 63 confirmed dengue pediatric patients recruited, hospital based longitudinal study detected six SD-SPL and ten dengue with warning sign (DWS). To identify the specific proteins increased or decreased in the SD-SPL plasma obtained ...[more]

PMID: 26895439

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A Proteomic Approach Identifies Candidate Early Biomarkers to Predict Severe Dengue in Children.

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A Proteomic Approach Identifies Candidate Early Biomarkers to Predict Severe Dengue in Children.

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