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Drosophila p24 and Sec22 regulate Wingless trafficking in the early secretory pathway.

ABSTRACT: The Wnt signaling pathway is crucial for development and disease. The regulation of Wnt protein trafficking is one of the pivotal issues in the Wnt research field. Here we performed a genetic screen in Drosophila melanogaster for genes involved in Wingless/Wnt secretion, and identified the p24 protein family members Baiser, CHOp24, Eclair and a v-SNARE protein Sec22, which are involved in the early secretory pathway of Wingless/Wnt. We provided genetic evidence demonstrating that loss of p24 proteins or Sec22 impedes Wingless (Wg) secretion in Drosophila wing imaginal discs. We found that Baiser cannot replace other p24 proteins (CHOp24 or Eclair) in escorting Wg, and only Baiser and CHOp24 interact with Wg. Moreover, we showed that the v-SNARE protein Sec22 and Wg are packaged together with p24 proteins. Taken together, our data provide important insights into the early secretory pathway of Wg/Wnt.

SUBMITTER: Li X

PROVIDER: S-EPMC4764680 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Drosophila p24 and Sec22 regulate Wingless trafficking in the early secretory pathway.

Li Xue X Wu Yihui Y Shen Chenghao C Belenkaya Tatyana Y TY Ray Lorraine L Lin Xinhua X

Biochemical and biophysical research communications 20150520 4

The Wnt signaling pathway is crucial for development and disease. The regulation of Wnt protein trafficking is one of the pivotal issues in the Wnt research field. Here we performed a genetic screen in Drosophila melanogaster for genes involved in Wingless/Wnt secretion, and identified the p24 protein family members Baiser, CHOp24, Eclair and a v-SNARE protein Sec22, which are involved in the early secretory pathway of Wingless/Wnt. We provided genetic evidence demonstrating that loss of p24 pro ...[more]

PMID: 26002470

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