Polyclonal Antibody Production for Membrane Proteins via Genetic Immunization.
Ontology highlight
ABSTRACT:
SUBMITTER: Hansen DT
PROVIDER: S-EPMC4764931 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Publications
Polyclonal Antibody Production for Membrane Proteins via Genetic Immunization.
Hansen Debra T DT Robida Mark D MD Craciunescu Felicia M FM Loskutov Andrey V AV Dörner Katerina K Rodenberry John-Charles JC Wang Xiao X Olson Tien L TL Patel Hetal H Fromme Petra P Sykes Kathryn F KF
Scientific reports 20160224
Antibodies are essential for structural determinations and functional studies of membrane proteins, but antibody generation is limited by the availability of properly-folded and purified antigen. We describe the first application of genetic immunization to a structurally diverse set of membrane proteins to show that immunization of mice with DNA alone produced antibodies against 71% (n = 17) of the bacterial and viral targets. Antibody production correlated with prior reports of target immunogen ...[more]
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