One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle.
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ABSTRACT:
SUBMITTER: Garrels W
PROVIDER: S-EPMC4764937 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Publications
One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle.
Garrels Wiebke W Talluri Thirumala R TR Apfelbaum Ronja R Carratalá Yanet P YP Bosch Pablo P Pötzsch Kerstin K Grueso Esther E Ivics Zoltán Z Kues Wilfried A WA
Scientific reports 20160224
Genetically modified cattle are important for developing new biomedical models and for an improved understanding of the pathophysiology of zoonotic diseases. However, genome editing and genetic engineering based on somatic cell nuclear transfer suffer from a low overall efficiency. Here, we established a highly efficient one-step multiplex gene transfer system into the bovine genome. ...[more]
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