Ontology highlight
ABSTRACT:
SUBMITTER: Martin K
PROVIDER: S-EPMC4766498 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Martin Katrin K Reimann Andreas A Fritz Rafael D RD Ryu Hyunryul H Jeon Noo Li NL Pertz Olivier O
Scientific reports 20160225
The three canonical Rho GTPases RhoA, Rac1 and Cdc42 co-ordinate cytoskeletal dynamics. Recent studies indicate that all three Rho GTPases are activated at the leading edge of motile fibroblasts, where their activity fluctuates at subminute time and micrometer length scales. Here, we use a microfluidic chip to acutely manipulate fibroblast edge dynamics by applying pulses of platelet-derived growth factor (PDGF) or the Rho kinase inhibitor Y-27632 (which lowers contractility). This induces acute ...[more]