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Highly multiplexed simultaneous detection of RNAs and proteins in single cells.

ABSTRACT: To enable the detection of expression signatures specific to individual cells, we developed PLAYR (proximity ligation assay for RNA), a method for highly multiplexed transcript quantification by flow and mass cytometry that is compatible with standard antibody staining. When used with mass cytometry, PLAYR allowed for the simultaneous quantification of more than 40 different mRNAs and proteins. In primary cells, we quantified multiple transcripts, with the identity and functional state of each analyzed cell defined on the basis of the expression of a separate set of transcripts or proteins. By expanding high-throughput deep phenotyping of cells beyond protein epitopes to include RNA expression, PLAYR opens a new avenue for the characterization of cellular metabolism.

SUBMITTER: Frei AP

PROVIDER: S-EPMC4767631 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Highly multiplexed simultaneous detection of RNAs and proteins in single cells.

Frei Andreas P AP Bava Felice-Alessio FA Zunder Eli R ER Hsieh Elena W Y EW Chen Shih-Yu SY Nolan Garry P GP Gherardini Pier Federico PF

Nature methods 20160125 3

To enable the detection of expression signatures specific to individual cells, we developed PLAYR (proximity ligation assay for RNA), a method for highly multiplexed transcript quantification by flow and mass cytometry that is compatible with standard antibody staining. When used with mass cytometry, PLAYR allowed for the simultaneous quantification of more than 40 different mRNAs and proteins. In primary cells, we quantified multiple transcripts, with the identity and functional state of each a ...[more]

PMID: 26808670

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