Ontology highlight
ABSTRACT:
SUBMITTER: Weinberg DE
PROVIDER: S-EPMC4767672 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Weinberg David E DE Shah Premal P Eichhorn Stephen W SW Hussmann Jeffrey A JA Plotkin Joshua B JB Bartel David P DP
Cell reports 20160211 7
Ribosome-footprint profiling provides genome-wide snapshots of translation, but technical challenges can confound its analysis. Here, we use improved methods to obtain ribosome-footprint profiles and mRNA abundances that more faithfully reflect gene expression in Saccharomyces cerevisiae. Our results support proposals that both the beginning of coding regions and codons matching rare tRNAs are more slowly translated. They also indicate that emergent polypeptides with as few as three basic residu ...[more]