Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes.
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ABSTRACT: Objective
This study evaluated in vitro differentiation of mesenchymal stromal cells isolated from bone marrow, in tenocytes after treatment with bovine tendon extract.Methods
Bovine tendons were used for preparation of the extract and were stored at -80 °C. Mesenchymal stromal cells from the bone marrow of three donors were used for cytotoxicity tests by means of MTT and cell differentiation by means of qPCR.Results
The data showed that mesenchymal stromal cells from bone marrow treated for up to 21 days in the presence of bovine tendon extract diluted at diminishing concentrations (1:10, 1:50 and 1:250) promoted activation of biglycan, collagen type I and fibromodulin expression.Conclusion
Our results show that bovine tendon extract is capable of promoting differentiation of bone marrow stromal cells in tenocytes.
SUBMITTER: Augusto LM
PROVIDER: S-EPMC4767843 | biostudies-literature | 2016 Jan-Feb
REPOSITORIES: biostudies-literature
Publications
Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes.
Augusto Lívia Maria Mendonça LM Aguiar Diego Pinheiro DP Bonfim Danielle Cabral DC Dos Santos Cavalcanti Amanda A Casado Priscila Ladeira PL Duarte Maria Eugênia Leite ME
Revista brasileira de ortopedia 20160106 1
<h4>Objective</h4>This study evaluated in vitro differentiation of mesenchymal stromal cells isolated from bone marrow, in tenocytes after treatment with bovine tendon extract.<h4>Methods</h4>Bovine tendons were used for preparation of the extract and were stored at -80 °C. Mesenchymal stromal cells from the bone marrow of three donors were used for cytotoxicity tests by means of MTT and cell differentiation by means of qPCR.<h4>Results</h4>The data showed that mesenchymal stromal cells from bon ...[more]
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