Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes.
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ABSTRACT:
SUBMITTER: Augusto LM
PROVIDER: S-EPMC4767843 | biostudies-literature | 2016 Jan-Feb
REPOSITORIES: biostudies-literature
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Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes.
Augusto Lívia Maria Mendonça LM Aguiar Diego Pinheiro DP Bonfim Danielle Cabral DC Dos Santos Cavalcanti Amanda A Casado Priscila Ladeira PL Duarte Maria Eugênia Leite ME
Revista brasileira de ortopedia 20160106 1
<h4>Objective</h4>This study evaluated in vitro differentiation of mesenchymal stromal cells isolated from bone marrow, in tenocytes after treatment with bovine tendon extract.<h4>Methods</h4>Bovine tendons were used for preparation of the extract and were stored at -80 °C. Mesenchymal stromal cells from the bone marrow of three donors were used for cytotoxicity tests by means of MTT and cell differentiation by means of qPCR.<h4>Results</h4>The data showed that mesenchymal stromal cells from bon ...[more]
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