Dataset Information

Genetic diversity and population structure of Polistes nimpha based on DNA microsatellite markers.

ABSTRACT: The Eurasiatic Polistes nimpha belongs to primitively eusocial wasps for which no data are available on its population's genetic structure and relatedness/relationships of individuals. The purpose of this research is to determine the amplification efficiency in P. nimpha of microsatellite primers developed for P. dominula and using these primers, to explore genetic diversity, population structure and relatedness/relationship of P. nimpha in the context of its reproductive options. Eight out of twelve microsatellite markers analyzed on 59 individuals (pupae and larvae) were polymorphic (mean PIC = 0.545) and mutated following the stepwise mutation model. The Bayesian clustering method gave the probability of >0.898 of there being 10 clusters within the pooled sample of 15 nests. In two or three nest clusters, full- and/or half-siblings and unrelated individuals occurred. A significant correlation between genetic and geographic distances was detected. There are three main possibilities that come into play to explain our genetic results and direct field observations: cooperative nest foundation, visitations, and/or usurpation events. So far there is no conclusive evidence to exclude or support any of these possibilities.

SUBMITTER: Kozyra KB

PROVIDER: S-EPMC4768218 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Genetic diversity and population structure of Polistes nimpha based on DNA microsatellite markers.

Kozyra K B KB Melosik I I Baraniak E E

Insectes sociaux 20150707

The Eurasiatic Polistes nimpha belongs to primitively eusocial wasps for which no data are available on its population's genetic structure and relatedness/relationships of individuals. The purpose of this research is to determine the amplification efficiency in P. nimpha of microsatellite primers developed for P. dominula and using these primers, to explore genetic diversity, population structure and relatedness/relationship of P. nimpha in the context of its reproduc ...[more]

PMID: 27034509

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Project description:The village and street dogs represent a unique model of canine populations. In the absence of selective breeding and veterinary care, they are subject mostly to natural selection. Their analyses contribute to understanding general mechanisms governing the genetic diversity, evolution and adaptation. In this study, we analyzed the genetic diversity and population structure of African village dogs living in villages in three different geographical areas in Northern Kenya. Data obtained for neutral microsatellite molecular markers were compared with those computed for potentially non-neutral markers of candidate immunity-related genes. The neutral genetic diversity was similar to other comparable village dog populations studied so far. The overall genetic diversity in microsatellites was higher than the diversity of European pure breeds, but it was similar to the range of diversity observed in a group composed of many European breeds, indicating that the African population has maintained a large proportion of the genetic diversity of the canine species as a whole. Microsatellite marker diversity indicated that the entire population is subdivided into three genetically distinct, although closely related subpopulations. This genetical partitioning corresponded to their geographical separation and the observed gene flow well correlated with the communication patterns among the three localities. In contrast to neutral microsatellites, the genetic diversity in immunity-related candidate SNP markers was similar across all three subpopulations and to the European group. It seems that the genetic structure of this particular population of Kenyan village dogs is mostly determined by geographical and anthropogenic factors influencing the gene flow between various subpopulations rather than by biological factors, such as genetic contribution of original migrating populations and/or the pathogen-mediated selection. On the other hand, the study of oldest surviving dogs suggested a biological mechanism, i.e. a possible advantage of the overal heterozygosity marked by the the microsatellite loci analyzed.

Project description:BackgroundIn Ethiopia, livestock contributes 45% of agricultural GDP. Despite the economic role played by the sector, there have been little efforts to genetically improve the indigenous cattle. Morphological characterization of selected Ethiopian indigenous cattle has been made for (Bonga, Jimma, and Kerayu) cattle types. But, the selected indigenous cattle were not characterized at molecular level (genetic diversity information). Hence, this work was initiated to detect and determine the genetic diversity and population structure of selected Ethiopian indigenous cattle ecotypes using microsatellite markers.ResultsDifferent alleles were identified (131) and thirty-three of these alleles were unique to specific ecotypes. All loci used were informative with PIC values ranging from 0.5 (TGLA126) to 0.84 (ETH10) with a mean of 0.70 per locus. The Shannon information index ranged from (I = 1.02) ILST006 to (I = 1.63) ETH10 with an average of 1.28 revealing there is genetic diversity. Moreover, analysis of molecular variance (AMOVA) revealed 84% genetic variation within a population and 13% variation among populations. The value of F-statistics (Fst) (0.129 = 13%) indicated that there was moderate genetic differentiation among ecotypes. The (UPGMA) revealed, Bonga and Jimma clustered together while Kerayu cattle were relatively distinct, Principal coordinates analysis (PCOA) and structure analysis grouped the individual into different clusters confirming the presence of ecotype admixture due to geographical origins and uncontrolled mating.ConclusionIn general, this study has successfully characterized the genetic diversity and population structure of Bonga, Jimma, and Kerayu cattle ecotypes using high polymorphic/informative microsatellite markers. According to this study, Kerayu cattle have high AR and PA when compared to Bonga and Jimma cattle populations. So, the Kerayu population is more diverse than others and it is the hotspot for genetic diversity study. The generated information is very relevant for breeder and genetic conservation.

Dataset Information

Genetic diversity and population structure of Polistes nimpha based on DNA microsatellite markers.

Publications

Genetic diversity and population structure of <i>Polistes nimpha</i> based on DNA microsatellite markers.

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