Ontology highlight
ABSTRACT:
SUBMITTER: Sampaziotis F
PROVIDER: S-EPMC4768345 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Nature biotechnology 20150713 8
The study of biliary disease has been constrained by a lack of primary human cholangiocytes. Here we present an efficient, serum-free protocol for directed differentiation of human induced pluripotent stem cells into cholangiocyte-like cells (CLCs). CLCs show functional characteristics of cholangiocytes, including bile acids transfer, alkaline phosphatase activity, γ-glutamyl-transpeptidase activity and physiological responses to secretin, somatostatin and vascular endothelial growth factor. We ...[more]