Ontology highlight
ABSTRACT:
SUBMITTER: Huan YJ
PROVIDER: S-EPMC4768780 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
The Journal of reproduction and development 20151103 1
Nuclear reprogramming induced by somatic cell nuclear transfer is an inefficient process, and donor cell DNA methylation status is thought to be a major factor affecting cloning efficiency. Here, the role of donor cell DNA methylation status regulated by 5-aza-2'-deoxycytidine (5-aza-dC) or 5-methyl-2'-deoxycytidine-5'-triphosphate (5-methyl-dCTP) in the early development of porcine cloned embryos was investigated. Our results showed that 5-aza-dC or 5-methyl-dCTP significantly reduced or increa ...[more]