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Alteration of the DNA methylation status of donor cells impairs the developmental competence of porcine cloned embryos.


ABSTRACT: Nuclear reprogramming induced by somatic cell nuclear transfer is an inefficient process, and donor cell DNA methylation status is thought to be a major factor affecting cloning efficiency. Here, the role of donor cell DNA methylation status regulated by 5-aza-2'-deoxycytidine (5-aza-dC) or 5-methyl-2'-deoxycytidine-5'-triphosphate (5-methyl-dCTP) in the early development of porcine cloned embryos was investigated. Our results showed that 5-aza-dC or 5-methyl-dCTP significantly reduced or increased the global methylation levels and altered the methylation and expression levels of key genes in donor cells. However, the development of cloned embryos derived from these cells was reduced. Furthermore, disrupted pseudo-pronucleus formation and transcripts of early embryo development-related genes were observed in cloned embryos derived from these cells. In conclusion, our results demonstrated that alteration of the DNA methylation status of donor cells by 5-aza-dC or 5-methyl-dCTP disrupted nuclear reprogramming and impaired the developmental competence of porcine cloned embryos.

SUBMITTER: Huan YJ 

PROVIDER: S-EPMC4768780 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Alteration of the DNA methylation status of donor cells impairs the developmental competence of porcine cloned embryos.

Huan Yan Jun YJ   Wu Zhan Feng ZF   Zhang Ji Guang JG   Zhu Jiang J   Xie Bing Teng BT   Wang Jian Yu JY   Li Jing Yu JY   Xue Bing Hua BH   Kong Qing Ran QR   Liu Zhong Hua ZH  

The Journal of reproduction and development 20151103 1


Nuclear reprogramming induced by somatic cell nuclear transfer is an inefficient process, and donor cell DNA methylation status is thought to be a major factor affecting cloning efficiency. Here, the role of donor cell DNA methylation status regulated by 5-aza-2'-deoxycytidine (5-aza-dC) or 5-methyl-2'-deoxycytidine-5'-triphosphate (5-methyl-dCTP) in the early development of porcine cloned embryos was investigated. Our results showed that 5-aza-dC or 5-methyl-dCTP significantly reduced or increa  ...[more]

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