Ontology highlight
ABSTRACT:
SUBMITTER: Pelz B
PROVIDER: S-EPMC4770092 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Pelz Benjamin B Žoldák Gabriel G Zeller Fabian F Zacharias Martin M Rief Matthias M
Nature communications 20160224
Enzymes are molecular machines that bind substrates specifically, provide an adequate chemical environment for catalysis and exchange products rapidly, to ensure fast turnover rates. Direct information about the energetics that drive conformational changes is difficult to obtain. We used subnanometre single-molecule force spectroscopy to study the energetic drive of substrate-dependent lid closing in the enzyme adenylate kinase. Here we show that in the presence of the bisubstrate inhibitor diad ...[more]