Ontology highlight
ABSTRACT:
SUBMITTER: Jin Y
PROVIDER: S-EPMC4770445 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Angewandte Chemie (International ed. in English) 20160128 10
Molecular details for RhoA/GAP catalysis of the hydrolysis of GTP to GDP are poorly understood. We use (19)F NMR chemical shifts in the MgF3(-) transition state analogue (TSA) complex as a spectroscopic reporter to indicate electron distribution for the γ-PO3(-) oxygens in the corresponding TS, implying that oxygen coordinated to Mg has the greatest electron density. This was validated by QM calculations giving a picture of the electronic properties of the transition state (TS) for nucleophilic ...[more]