A Comparative Study of New Aspergillus Strains for Proteolytic Enzymes Production by Solid State Fermentation.

ABSTRACT: A comparative study of the proteolytic enzymes production using twelve Aspergillus strains previously unused for this purpose was performed by solid state fermentation. A semiquantitative and quantitative evaluation of proteolytic activity were carried out using crude enzymatic extracts obtained from the fermentation cultures, finding seven strains with high and intermediate level of protease activity. Biochemical, thermodynamics, and kinetics features such as optimum pH and temperature values, thermal stability, activation energy (E a), quotient energy (Q 10), K m , and V max were studied in four enzymatic extracts from the selected strains that showed the highest productivity. Additionally, these strains were evaluated by zymogram analysis obtaining protease profiles with a wide range of

SUBMITTER: Ortiz GE 

PROVIDER: S-EPMC4771904 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature