Ontology highlight
ABSTRACT:
SUBMITTER: Diao Y
PROVIDER: S-EPMC4772021 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Diao Yarui Y Li Bin B Meng Zhipeng Z Jung Inkyung I Lee Ah Young AY Dixon Jesse J Maliskova Lenka L Guan Kun-Liang KL Shen Yin Y Ren Bing B
Genome research 20160126 3
With <2% of the human genome coding for proteins, a major challenge is to interpret the function of the noncoding DNA. Millions of regulatory sequences have been predicted in the human genome through analysis of DNA methylation, chromatin modification, hypersensitivity to nucleases, and transcription factor binding, but few have been shown to regulate transcription in their native contexts. We have developed a high-throughput CRISPR/Cas9-based genome-editing strategy and used it to interrogate 1 ...[more]