Characterizing transcriptional heterogeneity through pathway and gene set overdispersion analysis.
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ABSTRACT:
SUBMITTER: Fan J
PROVIDER: S-EPMC4772672 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Publications
Characterizing transcriptional heterogeneity through pathway and gene set overdispersion analysis.
Fan Jean J Salathia Neeraj N Liu Rui R Kaeser Gwendolyn E GE Yung Yun C YC Herman Joseph L JL Kaper Fiona F Fan Jian-Bing JB Zhang Kun K Chun Jerold J Kharchenko Peter V PV
Nature methods 20160118 3
The transcriptional state of a cell reflects a variety of biological factors, from cell-type-specific features to transient processes such as the cell cycle, all of which may be of interest. However, identifying such aspects from noisy single-cell RNA-seq data remains challenging. We developed pathway and gene set overdispersion analysis (PAGODA) to resolve multiple, potentially overlapping aspects of transcriptional heterogeneity by testing gene sets for coordinated variability among measured c ...[more]
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