Ontology highlight
ABSTRACT:
SUBMITTER: Ahrberg CD
PROVIDER: S-EPMC4773913 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Ahrberg Christian D CD Ilic Bojan Robert BR Manz Andreas A Neužil Pavel P
Lab on a chip 20160201 3
Here we report one of the smallest real-time polymerase chain reaction (PCR) systems to date with an approximate size of 100 mm × 60 mm × 33 mm. The system is an autonomous unit requiring an external 12 V power supply. Four simultaneous reactions are performed in the form of virtual reaction chambers (VRCs) where a ≈200 nL sample is covered with mineral oil and placed on a glass cover slip. Fast, 40 cycle amplification of an amplicon from the H7N9 gene was used to demonstrate the PCR performance ...[more]