Ontology highlight
ABSTRACT:
SUBMITTER: Wang P
PROVIDER: S-EPMC4775406 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Wang Peilu P Bahreini Amir A Gyanchandani Rekha R Lucas Peter C PC Hartmaier Ryan J RJ Watters Rebecca J RJ Jonnalagadda Amruth R AR Trejo Bittar Humberto E HE Berg Aaron A Hamilton Ronald L RL Kurland Brenda F BF Weiss Kurt R KR Mathew Aju A Leone Jose Pablo JP Davidson Nancy E NE Nikiforova Marina N MN Brufsky Adam M AM Ambros Tadeu F TF Stern Andrew M AM Puhalla Shannon L SL Lee Adrian V AV Oesterreich Steffi S
Clinical cancer research : an official journal of the American Association for Cancer Research 20151023 5
<h4>Purpose</h4>Given the clinical relevance of ESR1 mutations as potential drivers of resistance to endocrine therapy, this study used sensitive detection methods to determine the frequency of ESR1 mutations in primary and metastatic breast cancer, and in cell-free DNA (cfDNA).<h4>Experimental design</h4>Six ESR1 mutations (K303R, S463P, Y537C, Y537N, Y537S, D538G) were assessed by digital droplet PCR (ddPCR), with lower limits of detection of 0.05% to 0.16%, in primary tumors (n = 43), bone (n ...[more]