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A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics.

ABSTRACT: Cryogenic microscopy methods have gained increasing popularity, as they offer an unaltered view on the architecture of biological specimens. As a prerequisite, samples must be handled under cryogenic conditions below their recrystallization temperature, and contamination during sample transfer and handling must be prevented. We present a high-vacuum cryo-transfer system that streamlines the entire handling of frozen-hydrated samples from the vitrification process to low temperature imaging for scanning transmission electron microscopy and transmission electron microscopy. A template for cryo-electron microscopy and multimodal cryo-imaging approaches with numerous sample transfer steps is presented.

SUBMITTER: Tacke S

PROVIDER: S-EPMC4776042 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics.

Tacke Sebastian S Krzyzanek Vladislav V Nüsse Harald H Wepf Roger Albert RA Klingauf Jürgen J Reichelt Rudolf R

Biophysical journal 20160201 4

Cryogenic microscopy methods have gained increasing popularity, as they offer an unaltered view on the architecture of biological specimens. As a prerequisite, samples must be handled under cryogenic conditions below their recrystallization temperature, and contamination during sample transfer and handling must be prevented. We present a high-vacuum cryo-transfer system that streamlines the entire handling of frozen-hydrated samples from the vitrification process to low temperature imaging for s ...[more]

PMID: 26910419

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