Unknown

Dataset Information

0

A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics.


ABSTRACT: Cryogenic microscopy methods have gained increasing popularity, as they offer an unaltered view on the architecture of biological specimens. As a prerequisite, samples must be handled under cryogenic conditions below their recrystallization temperature, and contamination during sample transfer and handling must be prevented. We present a high-vacuum cryo-transfer system that streamlines the entire handling of frozen-hydrated samples from the vitrification process to low temperature imaging for scanning transmission electron microscopy and transmission electron microscopy. A template for cryo-electron microscopy and multimodal cryo-imaging approaches with numerous sample transfer steps is presented.

SUBMITTER: Tacke S 

PROVIDER: S-EPMC4776042 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7815152 | biostudies-literature
| S-EPMC3566860 | biostudies-literature
| S-EPMC4699365 | biostudies-literature
| S-EPMC10148829 | biostudies-literature
| S-EPMC3303806 | biostudies-literature
| S-EPMC3659406 | biostudies-literature
| S-EPMC6163078 | biostudies-literature
| S-EPMC3261350 | biostudies-literature
| S-EPMC3465359 | biostudies-other
| S-EPMC3131499 | biostudies-literature