Ontology highlight
ABSTRACT:
SUBMITTER: Jain V
PROVIDER: S-EPMC4776209 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Jain Vaibhav V Plaisance-Bonstaff Karlie K Sangani Rajnikumar R Lanier Curtis C Dolce Alexander A Hu Jianhong J Brulois Kevin K Haecker Irina I Turner Peter P Renne Rolf R Krueger Brian B
Viruses 20160219 2
Kaposi's sarcoma-associated herpesvirus (KSHV) encodes 12 viral microRNAs (miRNAs) that are expressed during latency. Research into KSHV miRNA function has suffered from a lack of genetic systems to study viral miRNA mutations in the context of the viral genome. We used the Escherichia coli Red recombination system together with a new bacmid background, BAC16, to create mutants for all known KSHV miRNAs. The specific miRNA deletions or mutations and the integrity of the bacmids have been strictl ...[more]