Ontology highlight
ABSTRACT:
SUBMITTER: Venkatachalam V
PROVIDER: S-EPMC4776525 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Venkatachalam Vivek V Ji Ni N Wang Xian X Clark Christopher C Mitchell James Kameron JK Klein Mason M Tabone Christopher J CJ Florman Jeremy J Ji Hongfei H Greenwood Joel J Chisholm Andrew D AD Srinivasan Jagan J Alkema Mark M Zhen Mei M Samuel Aravinthan D T AD
Proceedings of the National Academy of Sciences of the United States of America 20151228 8
We present an imaging system for pan-neuronal recording in crawling Caenorhabditis elegans. A spinning disk confocal microscope, modified for automated tracking of the C. elegans head ganglia, simultaneously records the activity and position of ∼80 neurons that coexpress cytoplasmic calcium indicator GCaMP6s and nuclear localized red fluorescent protein at 10 volumes per second. We developed a behavioral analysis algorithm that maps the movements of the head ganglia to the animal's posture and l ...[more]