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Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron - effect of surface modification.

ABSTRACT: In this work, silica nanofibers (SNFs) were prepared by an electrospinning method and modified with poly-d-lysine (PDL) or (3-aminopropyl) trimethoxysilane (APTS) making biocompatible and degradable substrates for neuronal growth. The as-prepared SNF, modified SNF-PDL, and SNF-APTS were evaluated using scanning electron microscopy, nitrogen adsorption/desorption isotherms, contact angle measurements, and inductively coupled plasma atomic emission spectroscopy. Herein, the scanning electron microscopic images revealed that dissolution occurred in a corrosion-like manner by enlarging porous structures, which led to loss of structural integrity. In addition, covalently modified SNF-APTS with more hydrophobic surfaces and smaller surface areas resulted in significantly slower dissolution compared to SNF and physically modified SNF-PDL, revealing that different surface modifications can be used to tune the dissolution rate. Growth of primary hippocampal neuron on all substrates led to a slower dissolution rate. The three-dimensional SNF with larger surface area and higher surface density of the amino group promoted better cell attachment and resulted in an increased neurite density. This is the first known work addressing the degradability of SNF substrate in physiological conditions with neuron growth in vitro, suggesting a strong potential for the applications of the material in controlled drug release.

SUBMITTER: Feng ZV

PROVIDER: S-EPMC4777233 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron - effect of surface modification.

Feng Z Vivian ZV Chen Wen Shuo WS Keratithamkul Khomson K Stoick Michael M Kapala Brittany B Johnson Eryn E Huang An-Chi AC Chin Ting Yu TY Chen-Yang Yui Whei YW Yang Mong-Lin ML

International journal of nanomedicine 20160226

In this work, silica nanofibers (SNFs) were prepared by an electrospinning method and modified with poly-d-lysine (PDL) or (3-aminopropyl) trimethoxysilane (APTS) making biocompatible and degradable substrates for neuronal growth. The as-prepared SNF, modified SNF-PDL, and SNF-APTS were evaluated using scanning electron microscopy, nitrogen adsorption/desorption isotherms, contact angle measurements, and inductively coupled plasma atomic emission spectroscopy. Herein, the scanning electron micro ...[more]

PMID: 27013873

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Project description:BackgroundHere, electrospun fibers based on a blend of polycaprolactone (PCL), poly(ethylene glycol) (PEG), and gelatin methacryloyl (GelMA) were developed. The careful choice of this polymer combination allowed for the preparation of a biomaterial that preserved the mechanical strength of PCL, while at the same time improving the hydrophilicity of the blended material and human osteoblast maturation.MethodsThe morphology, chemical structure, wettability, and mechanical properties before and after UV photocrosslinking were evaluated. Furthermore, human osteoblasts (hFOB) were cultivated for up to 21 days on the scaffolds, and their potential to upregulate cell proliferation, alkaline phosphatase (ALP) activity, and calcium deposition were investigated.ResultsContact angle measurement results showed that the developed scaffolds presented hydrophilic properties after PEG and GelMA incorporation before (25°) and after UV photocross-linking (69°) compared to pure PCL (149°). PCL:PEG:GelMA-UV displayed a slight increase in mechanical strength (elastic modulus ~37 MPa) over PCL alone (~33 MPa). Normally, an increase in strength of fibers leads to a decrease in elongation at break, due to the material becoming less deformable and stiffer, thus leading to breaks at low strain. This behavior was observed by comparing PCL (elongation at break ~106%) and PCL:PEG:GelMA-UV (~50%). Moreover, increases in ALP activity (10-fold at day 14) and calcium deposition (1.3-fold at day 21) by hFOBs were detected after PEG and GelMA incorporation after UV photocross-linking compared to pure PCL. Ultrathin and hydrophilic fibers were obtained after PEG and GelMA incorporation after UV photocrosslinking, but the strength of PCL was maintained. Interestingly, those ultrathin fiber characteristics improved hFOB functions.ConclusionThese findings appear promising for the use of these electrospun scaffolds, based on the combination of polymers used here for numerous orthopedic applications.

Dataset Information

Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron - effect of surface modification.

Publications

Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron - effect of surface modification.

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