Ontology highlight
ABSTRACT:
SUBMITTER: Zhao H
PROVIDER: S-EPMC4778845 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Zhao Hongyu H Shen Ao A Xiang Yang K YK Corey David P DP
PloS one 20160304 3
We describe three recombinant engineered antibodies against three recombinant epitope tags, constructed with divalent binding arms to recognize divalent epitopes and so achieve high affinity and specificity. In two versions, an epitope is inserted in tandem into a protein of interest, and a homodimeric antibody is constructed by fusing a high-affinity epitope-binding domain to a human or mouse Fc domain. In a third, a heterodimeric antibody is constructed by fusing two different epitope-binding ...[more]