Dataset Information

Morphological and molecular characterization of Eimeria purpureicephali n. sp. (Apicomplexa:Eimeriidae) in a red-capped parrot (Purpureicephalus spurius, Kuhl, 1820) in Western Australia.

ABSTRACT: A new Eimeria species is described from a red-capped parrot (Purpureicephalus spurius). Sporulated oocysts (n = 31) were spherical to subspherical, with a rough bilayered oocyst wall 0.8 ?m thick. Oocysts measured 24.0 × 22.8 (20.4-26.4 × 18.3-25.9) ?m, oocyst length/width ratio, 1.10. Oocyst residuum, polar granule and micropyle were absent. Sporocysts are elongate-ovoid, 11.0 × 7.3 (12.7-9.2 × 7.9-6.6) ?m, sporocyst length/width ratio, 1.51 (1.33-1.71). The thin convex Stieda body and indistinct substieda bodies were present and the sporocyst residuum was composed of numerous small granules less than 1.0 ?m in diameter dispersed randomly. Each sporocyst contained 2 sausage-shaped sporozoites in head-to-tail arrangement. The sporozoite nuclei were located centrally surrounded by refractile bodies. Molecular analysis was conducted at two loci; the 18S ribosomal RNA gene and the cytochrome c oxidase subunit I gene. At the18S locus, the new isolate shared 99.0% genetic similarity with Eimeria dispersa and Eimeria innocua from the turkey. At the cytochrome c oxidase subunit I gene locus, this new isolate was most closely related to E. dispersa and E. innocua, presented 99.0% and 98.0% genetic similarity, respectively. This new isolate and E. dispersa grouped together in the same clade. Based on the morphological and molecular data, this isolate is a new species of coccidian parasite, which is named Eimeria purpureicephali n. sp. after its host, the red-capped parrot (Purpureicephalus spurius).

SUBMITTER: Yang R

PROVIDER: S-EPMC4781968 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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Morphological and molecular characterization of Eimeria purpureicephali n. sp. (Apicomplexa:Eimeriidae) in a red-capped parrot (Purpureicephalus spurius, Kuhl, 1820) in Western Australia.

Yang Rongchang R Brice Belinda B Ryan Una U

International journal for parasitology. Parasites and wildlife 20160112 1

A new Eimeria species is described from a red-capped parrot (Purpureicephalus spurius). Sporulated oocysts (n = 31) were spherical to subspherical, with a rough bilayered oocyst wall 0.8 μm thick. Oocysts measured 24.0 × 22.8 (20.4-26.4 × 18.3-25.9) μm, oocyst length/width ratio, 1.10. Oocyst residuum, polar granule and micropyle were absent. Sporocysts are elongate-ovoid, 11.0 × 7.3 (12.7-9.2 × 7.9-6.6) μm, sporocyst length/width ratio, 1.51 (1.33-1.71). The thin convex Stieda body and indistin ...[more]

PMID: 26977403

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Project description:BackgroundClinical and subclinical coccidiosis is cosmopolitan and inflicts significant losses to the poultry industry globally. Seven named Eimeria species are responsible for coccidiosis in turkeys: Eimeria dispersa; Eimeria meleagrimitis; Eimeria gallopavonis; Eimeria meleagridis; Eimeria adenoeides; Eimeria innocua; and, Eimeria subrotunda. Although attempts have been made to characterize these parasites molecularly at the nuclear 18S rDNA and ITS loci, the maternally-derived and mitotically replicating mitochondrial genome may be more suited for species level molecular work; however, only limited sequence data are available for Eimeria spp. infecting turkeys. The purpose of this study was to sequence and annotate the complete mitochondrial genomes from 5 Eimeria species that commonly infect the domestic turkey (Meleagris gallopavo).MethodsSix single-oocyst derived cultures of five Eimeria species infecting turkeys were PCR-amplified and sequenced completely prior to detailed annotation. Resulting sequences were aligned and used in phylogenetic analyses (BI, ML, and MP) that included complete mitochondrial genomes from 16 Eimeria species or concatenated CDS sequences from each genome.ResultsComplete mitochondrial genome sequences were obtained for Eimeria adenoeides Guelph, 6211 bp; Eimeria dispersa Briston, 6238 bp; Eimeria meleagridis USAR97-01, 6212 bp; Eimeria meleagrimitis USMN08-01, 6165 bp; Eimeria gallopavonis Weybridge, 6215 bp; and Eimeria gallopavonis USKS06-01, 6215 bp). The order, orientation and CDS lengths of the three protein coding genes (COI, COIII and CytB) as well as rDNA fragments encoding ribosomal large and small subunit rRNA were conserved among all sequences. Pairwise sequence identities between species ranged from 88.1% to 98.2%; sequence variability was concentrated within CDS or between rDNA fragments (where indels were common). No phylogenetic reconstruction supported monophyly of Eimeria species infecting turkeys; Eimeria dispersa may have arisen via host switching from another avian host. Phylogenetic analyses suggest E. necatrix and E. tenella are related distantly to other Eimeria of chickens.ConclusionsMitochondrial genomes of Eimeria species sequenced to date are highly conserved with regard to gene content and structure. Nonetheless, complete mitochondrial genome sequences and, particularly the three CDS, possess sufficient sequence variability for differentiating Eimeria species of poultry. The mitochondrial genome sequences are highly suited for molecular diagnostics and phylogenetics of coccidia and, potentially, genetic markers for molecular epidemiology.

Project description:Eimeria spp. infection was investigated in 10 free-roaming European bison aged three months to 26 years by anatomopathological, histopathological, coproscopic and PCR-RFLP examination. The coproscopic study identified Eimeria oocysts in the faeces of five bison. The most prevalent morphotypes were E. bovis, present in all positive samples, and E. zuernii, in all but one. Additionally, mixed infections consisting of E. bovis, E. zuernii, E. alabamensis, E. auburnensis, E. canadensis, E. cylindrica, E. ellipsoidalis and E. subspherica were diagnosed in two bison calves. Besides being the most prevalent form, E. bovis also demonstrated the highest OPG (2,750). The presence of oocysts in the faeces was associated with those of macrogamonts, microgamonts and oocysts in the epithelium of the large intestine. Intestinal coccidiosis associated with lymphoplasmacytic enteritis was observed in many bison, not only those with positive OPG. Four animals with negative coproscopy results demonstrated early-stage gametogony in the large intestine; one case presented no endogenous stages of coccidians in the histopathological sections of the intestine, nor oocysts in the faecal samples. A 530 bp product of E. bovis 18S rDNA (GenBank: MK951685) was obtained from both the colon wall and oocysts; this was subjected to PCR-RFLP analysis based on AluI and Hin1II (NlaIII) restriction enzymes. Both samples yielded a consistent seven-band pattern, four of which (270 bp, 40 bp, 180 bp and 84 bp) were expected, and the other three represented undigested fragments. The obtained digestion pattern is indicative of Eimeria spp. infection, and can serve as a first-step diagnostic approach in detection of infection. The result of computer-based virtual digestion of the PCR product suggests that double digestion with Mval (BstNI) and KpnI restriction enzymes may be used as a second-step tool to distinguish between E. bovis, E. zuernii and E. alabamensis, all of which are highly-pathogenic species.

Dataset Information

Morphological and molecular characterization of Eimeria purpureicephali n. sp. (Apicomplexa:Eimeriidae) in a red-capped parrot (Purpureicephalus spurius, Kuhl, 1820) in Western Australia.

Publications

Morphological and molecular characterization of Eimeria purpureicephali n. sp. (Apicomplexa:Eimeriidae) in a red-capped parrot (Purpureicephalus spurius, Kuhl, 1820) in Western Australia.

Similar Datasets

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