Proteomic characterization of circulating extracellular vesicles identifies novel serum myeloma associated markers.

ABSTRACT: Multiple myeloma (MM) is a hematological malignancy of clonal plasma cells in the bone marrow (BM). The microenvironment plays a key role in MM cell survival and drug resistance through release of soluble factors, expression of adhesion molecules and release of extracellular vesicles (EVs). The aim of this manuscript is to use proteomic profiling of EVs as a tool to identify circulating tumor associated markers in MM patients. First, we characterized the EV protein content obtained from different MM cell lines. Then, we established differences in protein abundance among EVs isolated from MM patient serum and BM and the serum of healthy donors. These data show that the Major Histocompatibility Complex Class I is highly enriched in EVs of MM cell lines and MM patient's serum. Next, we show t

SUBMITTER: Harshman SW 

PROVIDER: S-EPMC4783258 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature