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Radiation damage and derivatization in macromolecular crystallography: a structure factor's perspective.

ABSTRACT: During, or even after, data collection the presence and effects of radiation damage in macromolecular crystallography may not always be immediately obvious. Despite this, radiation damage is almost always present, with site-specific damage occurring on very short time (dose) scales well before global damage becomes apparent. A result of both site-specific radiation damage and derivatization is a change in the relative intensity of reflections. The size and approximate rate of onset of X-ray-induced transformations is compared with the changes expected from derivatization, and strategies for minimizing radiation damage are discussed.

SUBMITTER: Owen RL

PROVIDER: S-EPMC4784669 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Radiation damage and derivatization in macromolecular crystallography: a structure factor's perspective.

Owen Robin L RL Sherrell Darren A DA

Acta crystallographica. Section D, Structural biology 20160301 Pt 3

During, or even after, data collection the presence and effects of radiation damage in macromolecular crystallography may not always be immediately obvious. Despite this, radiation damage is almost always present, with site-specific damage occurring on very short time (dose) scales well before global damage becomes apparent. A result of both site-specific radiation damage and derivatization is a change in the relative intensity of reflections. The size and approximate rate of onset of X-ray-indu ...[more]

PMID: 26960125

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