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Early Developmental Program Shapes Colony Morphology in Bacteria.

ABSTRACT: When grown on a solid surface, bacteria form highly organized colonies, yet little is known about the earliest stages of colony establishment. Following Bacillus subtilis colony development from a single progenitor cell, a sequence of highly ordered spatiotemporal events was revealed. Colony was initiated by the formation of leading-cell chains, deriving from the colony center and extending in multiple directions, typically in a "Y-shaped" structure. By eradicating particular cells during these early stages, we could influence the shape of the resulting colony and demonstrate that Y-arm extension defines colony size. A mutant in ymdB encoding a phosphodiesterase displayed unordered developmental patterns, indicating a role in guiding these initial events. Finally, we provide evidence that intercellular nanotubes contribute to proper colony formation. In summary, we reveal a "construction plan" for building a colony and provide the initial molecular basis for this process.

SUBMITTER: Mamou G

PROVIDER: S-EPMC4785774 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Early Developmental Program Shapes Colony Morphology in Bacteria.

Mamou Gideon G Malli Mohan Ganesh Babu GB Rouvinski Alex A Rosenberg Alex A Ben-Yehuda Sigal S

Cell reports 20160218 8

When grown on a solid surface, bacteria form highly organized colonies, yet little is known about the earliest stages of colony establishment. Following Bacillus subtilis colony development from a single progenitor cell, a sequence of highly ordered spatiotemporal events was revealed. Colony was initiated by the formation of leading-cell chains, deriving from the colony center and extending in multiple directions, typically in a "Y-shaped" structure. By eradicating particular cells during these ...[more]

PMID: 26904951

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