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Different food sources elicit fast changes to bacterial virulence.

ABSTRACT: Environmentally transmitted, opportunistic bacterial pathogens have a life cycle that alternates between hosts and environmental reservoirs. Resources are often scarce and fluctuating in the outside-host environment, whereas overcoming the host immune system could allow pathogens to establish a new, resource abundant and stable niche within the host. We tested if short-term exposure to different outside-host resource types and concentrations affect Serratia marcescens-(bacterium)'s virulence in Galleria mellonella (moth). As expected, virulence was mostly dictated by the bacterial dose, but we also found a clear increase in virulence when the bacterium had inhabited a low (versus high) resource concentration, or animal-based (versus plant-based) resources for 48 h prior to injection. The results suggest that temporal changes in pathogen's resource environment can induce very rapid changes in virulence and affect infection severity. Such changes could also play an important role in shifts from environmental lifestyle to pathogenicity or switches in host range and have implications for the management of opportunistic pathogens and disease outbreaks.

SUBMITTER: Ketola T

PROVIDER: S-EPMC4785914 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:BACKGROUND:Some pathogenic bacteria have been developing as a part of terrestrial and aquatic microbial ecosystems. Bacteria are consumed by bacteriovorous protists which are readily consumed by larger organisms. Being natural predators, protozoa are also an instrument for selection of virulence traits in bacteria. Moreover, protozoa serve as a "Trojan horse" that deliver pathogens to the human body. Here, we suggested that carnivorous amoebas feeding on smaller bacteriovorous protists might serve as "Troy" themselves when pathogens are delivered to them with their preys. A dual role might be suggested for protozoa in the development of traits required for bacterial passage along the food chain. RESULTS:A model food chain was developed. Pathogenic bacteria L. monocytogenes or related saprophytic bacteria L. innocua constituted the base of the food chain, bacteriovorous ciliate Tetrahymena pyriformis was an intermediate consumer, and carnivorous amoeba Amoeba proteus was a consumer of the highest order. The population of A. proteus demonstrated variations in behaviour depending on whether saprophytic or virulent Listeria was used to feed the intermediate consumer, T. pyriformis. Feeding of A. proteus with T. pyriformis that grazed on saprophytic bacteria caused prevalence of pseudopodia-possessing hungry amoebas. Statistically significant prevalence of amoebas with spherical morphology typical for fed amoebas was observed when pathogenic L. monocytogenes were included in the food chain. Moreover, consumption of tetrahymenas fed with saprophytic L. innocua improved growth of A. proteus population while L. monocytogenes-filled tetrahymenas provided negative effect. Both pathogenic and saprophytic bacteria were delivered to A. proteus alive but only L. monocytogenes multiplied within amoebas. Observed differences in A. proteus population behaviour suggested that virulent L. monocytogenes might slow down restoration of A. proteus ability to hunt again and thus restrict the size of A. proteus population. Comparison of isogenic bacterial pairs that did or did not produce the haemolysin listeriolysin O (LLO) suggested a role for LLO in passing L. monocytogenes along the food chain. CONCLUSIONS:Our results support the idea of protozoa as a means of pathogen delivery to consumers of a higher order and demonstrated a dual role of protozoa as both a "Trojan horse" and "Troy."

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Different food sources elicit fast changes to bacterial virulence.

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