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A Versatile Strategy for Isolating a Highly Enriched Population of Intestinal Stem Cells.

ABSTRACT: The isolation of pure populations of mouse intestinal stem cells (ISCs) is essential to facilitate functional studies of tissue homeostasis, tissue regeneration, and intestinal diseases. However, the purification of ISCs has relied predominantly on the use of transgenic reporter alleles in mice. Here, we introduce a combinational cell surface marker-mediated strategy that allows the isolation of an ISC population transcriptionally and functionally equivalent to the gold standard Lgr5-GFP ISCs. Used on reporter-free mice, this strategy allows the isolation of functional, transcriptionally distinct ISCs uncompromised by Lgr5 haploinsufficiency.

SUBMITTER: Nefzger CM

PROVIDER: S-EPMC4788784 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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A Versatile Strategy for Isolating a Highly Enriched Population of Intestinal Stem Cells.

Nefzger Christian M CM Jardé Thierry T Rossello Fernando J FJ Horvay Katja K Knaupp Anja S AS Powell David R DR Chen Joseph J Abud Helen E HE Polo Jose M JM

Stem cell reports 20160225 3

The isolation of pure populations of mouse intestinal stem cells (ISCs) is essential to facilitate functional studies of tissue homeostasis, tissue regeneration, and intestinal diseases. However, the purification of ISCs has relied predominantly on the use of transgenic reporter alleles in mice. Here, we introduce a combinational cell surface marker-mediated strategy that allows the isolation of an ISC population transcriptionally and functionally equivalent to the gold standard Lgr5-GFP ISCs. U ...[more]

PMID: 26923822

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