Ontology highlight
ABSTRACT:
SUBMITTER: Roberts TC
PROVIDER: S-EPMC4790731 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Roberts Thomas C TC Hart Jonathan R JR Kaikkonen Minna U MU Weinberg Marc S MS Vogt Peter K PK Morris Kevin V KV
Nature protocols 20150716 8
Nuclear run-on (NRO) is a method that measures transcriptional activity via the quantification of biochemically labeled nascent RNA molecules derived from nuclear isolates. Widespread use of this technique has been limited because of its technical difficulty relative to steady-state total mRNA analyses. Here we describe a detailed protocol for the quantification of transcriptional activity in human cell cultures. Nuclei are first isolated and NRO transcription is performed in the presence of bro ...[more]