Improvement of the banana "Musa acuminata" reference sequence using NGS data and semi-automated bioinformatics methods.

ABSTRACT:

Background

Recent advances in genomics indicate functional significance of a majority of genome sequences and their long range interactions. As a detailed examination of genome organization and function requires very high quality genome sequence, the objective of this study was to improve reference genome assembly of banana (Musa acuminata).

Results

We have developed a modular bioinformatics pipeline to improve genome sequence assemblies, which can handle various types of data. The pipeline comprises several semi-automated tools. However, unlike classical automated tools that are based on global parameters, the semi-automated tools proposed an expert mode for a user who can decide on suggested improvements through local compromises. The pipeline was used to improve the draft

SUBMITTER: Martin G 

PROVIDER: S-EPMC4793746 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature